<p>Lymphoscintigraphy is widely considered to be the main investigation to establish the diagnosis of lymphoedema and visualise peripheral lymphatics. However, there is no internationally agreed standardised technique. This article considers technical aspects of lymphoscintigraphy (radiotracers used, sites of injection, qualitative and quantitative methods and the sensitivity and specificity of the technique) and its clinical application, including the indications for its use, the interpretation of the results obtained and clinical examples from the Derby lymphoedema service.</p>